StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman, Mouse
PurificationImmunogen affinity purified
ImmunogenATG4 autophagy related 4 homolog C (S. cerevisiae)
IHCImmunohistochemistry of paraffin-embedded human heart using A000675(ATG4C antibody) at dilution of 1:50
Western Blotmouse testis tissue were subjected to SDS PAGE followed by western blot with A000675(ATG4C antibody) at dilution of 1:500
Recommended dilutionWB : 1:200-1:2000
Product Description specificalCysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.