-
Alternative name
NIFUN antibody
-
Catalog
A004402
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat
-
Applications Tested/SuitableELISA,WB,IHC,IF,IP
-
PurificationImmunogen affinity purified
-
Immunogeniron-sulfur cluster scaffold homolog (E. coli)
-
IHCImmunohistochemistry of paraffin-embedded human lung cancer using A004402(ISCU antibody) at dilution of 1:50
-
Western Blotmouse heart tissue were subjected to SDS PAGE followed by western blot with A004402(ISCU antibody) at dilution of 1:500
-
Recommended dilutionWB : 1:200-1:2000
-
Product Description specificalScaffold protein for the de novo synthesis of iron- sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity).
-
Gene nameISCU
-
Uniprot IDQ9H1K1
-
Observed M.W.14-15 kDa,18-19 kDa
-