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Alternative name
DKFZp686F20131, Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP antibody
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Catalog
A009530
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IHC
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PurificationProtein A+G purification
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ImmunogenWilms tumor 1 associated protein
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IHCImmunohistochemistry of paraffin-embedded human cervical cancer using A009530(WTAP antibody) at dilution of 1:50
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A009530( WTAP Antibody) at dilution of 1:2000
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Recommended dilutionWB:1:1000-1:10000;IF:1:20-1:200;IHC:1:20-1:200
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Product Description specificalRegulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).
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Gene nameWTAP
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locationNucleus
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Uniprot IDQ15007
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Calculated M.W.49kDa
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Observed M.W.50-55 kDa
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