anti-BrdU antibody

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  • Alternative name

    5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR antibody

  • Catalog
  • Size100μg
  • Formliquid
  • Purity≥95% as determined by SDS-PAGE
  • StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
  • ClonalityMonoclonal Antibody
  • Host Mouse
  • Species ReactivityHuman, Mouse ,Rat
  • Applications Tested/SuitableELISA,IF,IHC
  • PurificationProtein A+G purification
  • Immunogencompound
  • IHCImmunohistochemistry of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using A000952( BrDu Antibody) at dilution of 1:400. Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
  • Recommended dilutionIHC:1:200-1:1000;IF:1:50-1:500
  • Product Description specificalBromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.

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