anti-6*His, His-Tag antibody

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  • Alternative name

    6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag, HISTIDINE TAGGED antibody

  • Catalog
  • Size100μg
  • Formliquid
  • Purity≥95% as determined by SDS-PAGE
  • StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
  • ClonalityMonoclonal Antibody
  • Host Mouse
  • Species ReactivityRecombinant protein
  • Applications Tested/SuitableELISA,WB,IP,IF
  • PurificationProtein A+G purification
  • Western BlotWestern blot of 6*His-tagged fusion protein with A000008(anti-6*HIS tag) at dilution of 1:5000.
  • Recommended dilutionWB : 1:1000-1:10000;IP : 1:1000-1:10000;IF : 1:20-1:100
  • Product Description specificalProtein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

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