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Alternative name
CREB2, TXREB antibody
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Catalog
A000663
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,IHC,WB,IF
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PurificationProtein A+G purification
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Immunogenactivating transcription factor 4 (tax-responsive enhancer element B67)
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IHCImmunohistochemistry of paraffin-embedded human breast cancer tissue slide using A000663( ATF4 Antibody) at dilution of 1:50 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western BlotHepG2 cells were subjected to SDS PAGE followed by western blot with A000663( ATF4 Antibody) at dilution of 1:2000
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors (PubMed:26086088).
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Gene nameATF4
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Uniprot IDP18848
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Observed M.W.50-58 kDa, 35-38 kDa
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