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Alternative name
B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1, PD-L1/CD274, Programmed death ligand 1 antibody
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Catalog
A006281
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman, Mouse ,Pig
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Applications Tested/SuitableELISA,WB,IHC，IF
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PurificationProtein A+G purification
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ImmunogenCD274 molecule
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IHCImmunohistochemistry of paraffin-embedded human tonsillitis tissue slide using A006281(PD-L1/CD274 Antibody) at dilution of 1:50 . heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western Blothuman skeletal muscle tissue were subjected to SDS PAGE followed by western blot with A006281( PD-L1/CD274 Antibody) at dilution of 1:1000
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Recommended dilutionWB:1:500-1:5000;IHC:1:100-1:500;IF:1:50-1:500
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Product Description specificalProgrammed cell death ligand 1(CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, the apparent molecular weight has been reported as 45-70 kDa, suggesting probable glycosylation (PMID: 18760278; 16493058).?
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Gene nameCD274
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Uniprot IDQ9NZQ7
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Calculated M.W.33kDa,observed 50kDa
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Observed M.W.50-56 kDa, 65-70 kDa
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