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Alternative name
CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1 antibody
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Catalog
A006066
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman,Mouse , Monkey
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Applications Tested/SuitableELISA,WB
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PurificationProtein A+G purification
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Immunogencyclin-dependent kinase inhibitor 1A (p21, Cip1)
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Western BlotMCF-7 cells were subjected to SDS PAGE followed by western blot with A006066( P21 Antibody) at dilution of 1:1000
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Recommended dilutionWB:1:500-1:5000
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Product Description specificalCDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.
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Gene nameCDKN1A
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locationCytoplasm,Nucleus
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Uniprot IDP38936
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Calculated M.W.21kDa
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Observed M.W.21 kDa
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