StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman, Mouse
PurificationProtein A+G purification
Immunogenneuron derived neurotrophic factor
IHCImmunohistochemistry of paraffin-embedded human cerebellum using A005661(NENF antibody) at dilution of 1:50
Western Blothuman brain tissue were subjected to SDS PAGE followed by western blot with A005661(NENF antibody) at dilution of 1:1000
Recommended dilutionWB : 1:500-1:5000
Product Description specificalActs as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (By similarity). Plays a role in the human tumorigenesis (PubMed:22748190).