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Alternative name
ERK, ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3 antibody
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Catalog
A002847
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IHC,IF
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PurificationProtein A+G purification
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Immunogenmitogen-activated protein kinase 3
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IHCImmunohistochemistry of paraffin-embedded human ovary tumor tissue slide using A002847( ERK1/2 Antibody) at dilution of 1:200. Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A002847( ERK1/2 Antibody) at dilution of 1:1000
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Recommended dilutionWB：1:500-1:5000;IP:1:1000-1:10000;IHC:1:100-1:400;IF:1:20-1:200
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Product Description specificalERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF.
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Gene nameMAPK3
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locationCytoplasm,ucleus
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Uniprot IDP27361
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Calculated M.W.44kDa，observed 38 kDa,43 kDa
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Observed M.W.38-44 kDa
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