StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Species ReactivityHuman, Mouse ,Rat
PurificationProtein A+G purification
Immunogeneukaryotic translation initiation factor 3, subunit D
IHCImmunohistochemistry of paraffin-embedded human brain using A002705(EIF3D antibody) at dilution of 1:50
Western BlotHepG2 cells were subjected to SDS PAGE followed by western blot with A002705(EIF3D antibody) at dilution of 1:1000
Recommended dilutionWB : 1:200-1:2000
Product Description specificalComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).