-
Alternative name
CUL 4A, CUL4A, cullin 4A, Cullin-4A antibody
-
Catalog
A002077
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityMonoclonal Antibody
-
Host Mouse
-
Species ReactivityHuman, Monkey, Mouse ,Rat
-
Applications Tested/SuitableELISA,WB,IHC,IP,IF
-
PurificationProtein A+G purification
-
Immunogencullin 4A
-
IHCImmunohistochemistry of paraffin-embedded human heart tissue slide using A002077( CUL4A Antibody) at dilution of 1:200
-
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A002077(CUL4A antibody) at dilution of 1:500
-
Recommended dilutionIHC : 1:20-1:200
-
Product Description specificalCore component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL.
-
Gene nameCUL4A
-
Uniprot IDQ13619
-