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Alternative name
CD146, MCAM, MUC18,Cell surface glycoprotein MUC18,Cell surface glycoprotein P1H12,Melanoma cell adhesion molecule,Melanoma-associated antigen A32,Melanoma-associated antigen MUC18
antibody
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Catalog
A001429
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB,IHC,FC
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PurificationProtein A+G purification
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Immunogenmelanoma cell adhesion molecule
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IHCImmunohistochemistry of paraffin-embedded human liver cancer tissue slide using A001429( CD146, MCAM Antibody) at dilution of 1:20
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Western BlotA375 cells were subjected to SDS PAGE followed by western blot with A001429(CD146, MCAM antibody) at dilution of 1:1000
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Recommended dilutionWB:1:500-1:5000;IHC:1:20-1:200
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Product Description specificalCD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677).
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Gene nameMCAM
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Uniprot IDP43121
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Calculated M.W.72kDa,Observed 120kDa
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Observed M.W.113-120 kDa
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