-
Alternative name
Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5 antibody
-
Catalog
A001294
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityMonoclonal Antibody
-
Host Mouse
-
Species ReactivityHuman
-
Applications Tested/SuitableELISA,IHC,WB,IF,IP
-
PurificationProtein A+G purification
-
Immunogencaspase 8, apoptosis-related cysteine peptidase
-
IHCImmunohistochemistry of paraffin-embedded human liver cancer tissue slide using A001294( Caspase 8 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
-
Western BlotHepG2 cells were subjected to SDS PAGE followed by western blot with A001294(CASP8 antibody) at dilution of 1:1000
-
Recommended dilutionWB:1:500-1:5000;IP:1:200-1:2000;IHC:1:100-1:400;IF:1:50-1:500
-
Product Description specificalCaspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. 66093-1-AP can recognize pro- and cleaved-caspase 8.
-
Gene nameCASP8
-
locationCytoplasm
-
Uniprot IDQ14790
-
Calculated M.W.30-60kDa
-
Observed M.W.50-55 kDa,25-32 kDa
-