Human F10 ELISA Kit

Alternative name
Human Stuart factor ELISA Kit;Human Stuart-Prower factor ELISA Kit;Human FX ELISA Kit;Human FXA ELISA Kit;Human coagulation factor X ELISA Kit;Human factor Xa ELISA Kit;Human prothrombinase ELISA Kit;
Catalog
E009847
species
Human
GeneF10
SpecificityThis assay has high sensitivity and excellent specificity for detection of Coagulation Factor X (F10). No significant cross-reactivity or interference between Coagulation Factor X (F10) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.09ng/mL.
Intended UseHuman F10 ELISA Kit allows for the in vitro quantitative determination of F10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor X (F10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor X (F10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor X (F10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor X (F10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Activated factor Xa heavy chain Protein information
Uniprot ID FA10_HUMAN
Uniprot AC P00742; Q14340;
UniGene Hs.361463;
GeneID 2159
KEGG hsa:2159;
Human Activated factor Xa heavy chain Protein SEQUENCE
SEQUENCE 488 AA; 54732 MW; F81D5746AF4797AF CRC64;
MGRPLHLVLL SASLAGLLLL GESLFIRREQ ANNILARVTR ANSFLEEMKK
GHLERECMEE TCSYEEAREV FEDSDKTNEF WNKYKDGDQC ETSPCQNQGK
CKDGLGEYTC TCLEGFEGKN CELFTRKLCS LDNGDCDQFC HEEQNSVVCS
CARGYTLADN GKACIPTGPY PCGKQTLERR KRSVAQATSS SGEAPDSITW
KPYDAADLDP TENPFDLLDF NQTQPERGDN NLTRIVGGQE CKDGECPWQA
LLINEENEGF CGGTILSEFY ILTAAHCLYQ AKRFKVRVGD RNTEQEEGGE
AVHEVEVVIK HNRFTKETYD FDIAVLRLKT PITFRMNVAP ACLPERDWAE
STLMTQKTGI VSGFGRTHEK GRQSTRLKML EVPYVDRNSC KLSSSFIITQ
NMFCAGYDTK QEDACQGDSG GPHVTRFKDT YFVTGIVSWG EGCARKGKYG
IYTKVTAFLK WIDRSMKTRG LPKAKSHAPE VITSSPLK

Category

Human F10 ELISA Kit

Human Stuart factor ELISA Kit;Human Stuart-Prower factor ELISA Kit;Human FX ELISA Kit;Human FXA ELISA Kit;Human coagulation factor X ELISA Kit;Human factor Xa ELISA Kit;Human prothrombinase ELISA Kit;