Human F10 ELISA Kit

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  • Alternative name

    Human Stuart factor ELISA Kit;Human Stuart-Prower factor ELISA Kit;Human FX ELISA Kit;Human FXA ELISA Kit;Human coagulation factor X ELISA Kit;Human factor Xa ELISA Kit;Human prothrombinase ELISA Kit;

  • Catalog
    E009847
  • species
    Human
  • GeneF10
  • Standard CurveHuman F10 ELISA Kit
  • Other Species Mouse F10 ELISA KitBovine F10 ELISA KitChicken F10 ELISA KitRabbit F10 ELISA KitRat F10 ELISA KitChicken Class I histocompatibility antigen, F10 alpha chain ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Coagulation Factor X (F10). No significant cross-reactivity or interference between Coagulation Factor X (F10) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.09ng/mL.
  • Intended UseHuman F10 ELISA Kit allows for the in vitro quantitative determination of F10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor X (F10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor X (F10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor X (F10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor X (F10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Activated factor Xa heavy chain Protein information
  • Uniprot ID FA10_HUMAN
  • Uniprot AC P00742; Q14340;
  • UniGene Hs.361463;
  • GeneID 2159
  • KEGG hsa:2159;
  • Human Activated factor Xa heavy chain Protein SEQUENCE
  • SEQUENCE 488 AA; 54732 MW; F81D5746AF4797AF CRC64;

    MGRPLHLVLL SASLAGLLLL GESLFIRREQ ANNILARVTR ANSFLEEMKK

    GHLERECMEE TCSYEEAREV FEDSDKTNEF WNKYKDGDQC ETSPCQNQGK

    CKDGLGEYTC TCLEGFEGKN CELFTRKLCS LDNGDCDQFC HEEQNSVVCS

    CARGYTLADN GKACIPTGPY PCGKQTLERR KRSVAQATSS SGEAPDSITW

    KPYDAADLDP TENPFDLLDF NQTQPERGDN NLTRIVGGQE CKDGECPWQA

    LLINEENEGF CGGTILSEFY ILTAAHCLYQ AKRFKVRVGD RNTEQEEGGE

    AVHEVEVVIK HNRFTKETYD FDIAVLRLKT PITFRMNVAP ACLPERDWAE

    STLMTQKTGI VSGFGRTHEK GRQSTRLKML EVPYVDRNSC KLSSSFIITQ

    NMFCAGYDTK QEDACQGDSG GPHVTRFKDT YFVTGIVSWG EGCARKGKYG

    IYTKVTAFLK WIDRSMKTRG LPKAKSHAPE VITSSPLK




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