Human F8 ELISA Kit

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  • Alternative name

    Coagulation factor VIII ELISA KIT; Procoagulant componentF8 ELISA KIT; Cf8 ELISA KIT; F8c ELISA KIT

  • Catalog
    E009835
  • species
    Human
  • GeneF8
  • Standard CurveHuman F8 ELISA Kit
  • Other Species Human F8 Ag ELISA KitHuman F8-AG ELISA KitMouse F8 ELISA KitMouse F8-Ag ELISA KitCanine F8 ELISA KitPorcine F8 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Coagulation Factor VIII (F8). No significant cross-reactivity or interference between Coagulation Factor VIII (F8) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.058ng/mL.
  • Intended UseHuman F8 ELISA Kit allows for the in vitro quantitative determination of F8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII (F8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor VIII (F8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor VIII (F8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor VIII (F8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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