Human KCNJ10 ELISA Kit

Alternative name
Human ATP-dependent inwardly rectifying potassium channel Kir4.1 ELISA Kit;Human inward rectifier K(+) channel Kir1.2 ELISA Kit;Human potassium channel, inwardly rectifying subfamily J member 10 ELISA Kit;Human BIRK-10 ELISA Kit;Human KCNJ13-PEN ELISA Kit;Human KIR1.2 ELISA Kit;Human KIR4.1 ELISA Kit;Human SESAME ELISA Kit;Human potassium voltage-gated channel subfamily J member 10 ELISA Kit;Human ATP-sensitive inward rectifier potassium channel 10 ELISA Kit;Human glial ATP-dependent inwardly rectifying potassium channel KIR4.1 ELISA Kit;Human inward rectifier K+ channel KIR1.2 ELISA Kit;Human potassium inwardly-rectifying channel, subfamily J, member 10 ELISA Kit;
Catalog
E004044
species
Human
GeneKCNJ10
Standard Curve
Other Species Mouse Kcnj10 ELISA Kit Rat Kcnj10 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of KCNJ10. No significant cross-reactivity or interference between KCNJ10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KCNJ10 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman KCNJ10 ELISA Kit allows for the in vitro quantitative determination of KCNJ10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This KCNJ10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KCNJ10. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||KCNJ10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KCNJ10 antibody and an KCNJ10-HRP conjugate. The assay sample and buffer are incubated together with KCNJ10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KCNJ10 concentration since KCNJ10 from samples and KCNJ10-HRP conjugate compete for the anti-KCNJ10 antibody binding site. Since the number of sites is limited, as more sites are occupied by KCNJ10 from the sample, fewer sites are left to bind KCNJ10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KCNJ10 concentration in each sample is interpolated from this standard curve.
Human ATP-sensitive inward rectifier potassium channel 10 Protein information
Uniprot ID KCJ10_HUMAN
Uniprot AC P78508; A3KME7; Q5VUT9; Q8N4I7; Q92808;
UniGene Hs.408960;
GeneID 3766
KEGG hsa:3766;
Human ATP-sensitive inward rectifier potassium channel 10 Protein SEQUENCE
SEQUENCE 379 AA; 42508 MW; D9DA013FF4003533 CRC64;
MTSVAKVYYS QTTQTESRPL MGPGIRRRRV LTKDGRSNVR MEHIADKRFL
YLKDLWTTFI DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELD
PPANHTPCVV QVHTLTGAFL FSLESQTTIG YGFRYISEEC PLAIVLLIAQ
LVLTTILEIF ITGTFLAKIA RPKKRAETIR FSQHAVVASH NGKPCLMIRV
ANMRKSLLIG CQVTGKLLQT HQTKEGENIR LNQVNVTFQV DTASDSPFLI
LPLTFYHVVD ETSPLKDLPL RSGEGDFELV LILSGTVEST SATCQVRTSY
LPEEILWGYE FTPAISLSAS GKYIADFSLF DQVVKVASPS GLRDSTVRYG
DPEKLKLEES LREQAEKEGS ALSVRISNV
UCSC uc001fuw.3; human.;

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Human KCNJ10 ELISA Kit