Human AK2 ELISA Kit

Contact Us
  • Alternative name

    Human AK 2 ELISA Kit;Human ATP-AMP transphosphorylase 2 ELISA Kit;Human ATP:AMP phosphotransferase ELISA Kit;Human adenylate monophosphate kinase ELISA Kit;Human ADK2 ELISA Kit;Human adenylate kinase 2 ELISA Kit;Human adenylate kinase 2, mitochondrial ELISA Kit;Human adenylate kinase isoenzyme 2, mitochondrial ELISA Kit;Human testis secretory sperm-binding protein Li 220n ELISA Kit;

  • Catalog
    E000867
  • species
    Human
  • GeneAK2
  • Standard CurveHuman AK2 ELISA Kit
  • Other Species Mouse Ak2 ELISA KitBovine AK2 ELISA KitRat Ak2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AK2. No significant cross-reactivity or interference between AK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AK2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman AK2 ELISA Kit allows for the in vitro quantitative determination of AK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: AK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AK2 antibody and an AK2-HRP conjugate. The assay sample and buffer are incubated together with AK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AK2 concentration since AK2 from samples and AK2-HRP conjugate compete for the anti-AK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AK2 from the sample, fewer sites are left to bind AK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AK2 concentration in each sample is interpolated from this standard curve.
  • Human Adenylate kinase 2, mitochondrial, N-terminally processed Protein information
  • Uniprot ID KAD2_HUMAN
  • Uniprot AC Q8TCY3;
  • UniGene Hs.470907;
  • GeneID 204
  • KEGG hsa:204;
  • Human Adenylate kinase 2, mitochondrial, N-terminally processed Protein SEQUENCE
  • SEQUENCE 239 AA; 26478 MW; 86FA94F9EE33629F CRC64;

    MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML

    RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG

    FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR

    SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY

    YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI

  • UCSC uc001bwo.3; human. [P54819-1];


Related products with Human AK2 ELISA Kit


tag

Inquiry About Human AK2 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!