Human AK2 ELISA Kit

Alternative name
Human AK 2 ELISA Kit;Human ATP-AMP transphosphorylase 2 ELISA Kit;Human ATP:AMP phosphotransferase ELISA Kit;Human adenylate monophosphate kinase ELISA Kit;Human ADK2 ELISA Kit;Human adenylate kinase 2 ELISA Kit;Human adenylate kinase 2, mitochondrial ELISA Kit;Human adenylate kinase isoenzyme 2, mitochondrial ELISA Kit;Human testis secretory sperm-binding protein Li 220n ELISA Kit;
Catalog
E000867
species
Human
GeneAK2
SpecificityThis assay has high sensitivity and excellent specificity for detection of AK2. No significant cross-reactivity or interference between AK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AK2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman AK2 ELISA Kit allows for the in vitro quantitative determination of AK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: AK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AK2 antibody and an AK2-HRP conjugate. The assay sample and buffer are incubated together with AK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AK2 concentration since AK2 from samples and AK2-HRP conjugate compete for the anti-AK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AK2 from the sample, fewer sites are left to bind AK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AK2 concentration in each sample is interpolated from this standard curve.
Human Adenylate kinase 2, mitochondrial, N-terminally processed Protein information
Uniprot ID KAD2_HUMAN
Uniprot AC Q8TCY3;
UniGene Hs.470907;
GeneID 204
KEGG hsa:204;
Human Adenylate kinase 2, mitochondrial, N-terminally processed Protein SEQUENCE
SEQUENCE 239 AA; 26478 MW; 86FA94F9EE33629F CRC64;
MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML
RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG
FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR
SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY
YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI
UCSC uc001bwo.3; human. [P54819-1];

Human AK2 ELISA Kit

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