Human CDK1 ELISA Kit

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  • Alternative name

    Human CDK1 ELISA Kit;Human cell division control protein 2 homolog ELISA Kit;Human cell division protein kinase 1 ELISA Kit;Human p34 protein kinase ELISA Kit;Human CDC2 ELISA Kit;Human CDC28A ELISA Kit;Human CDKN1 ELISA Kit;Human P34CDC2 ELISA Kit;Human cyclin dependent kinase 1 ELISA Kit;Human cyclin-dependent kinase 1 ELISA Kit;Human cell cycle controller CDC2 ELISA Kit;Human cell division cycle 2, G1 to S and G2 to M ELISA Kit;

  • Catalog
    E007588
  • species
    Human
  • GeneCDK1
  • Other Species Mouse Cdk1 ELISA KitBovine CDK1 ELISA KitChicken CDK1 ELISA KitRat Cdk1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CDK1. No significant cross-reactivity or interference between Human CDK1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CDK1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseHuman CDK1 ELISA Kit allows for the in vitro quantitative determination of CDK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDK1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CDK1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CDK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CDK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CDK1. You can calculate the concentration of Human CDK1 in the samples by comparing the OD of the samples to the standard curve.
  • Human Cyclin-dependent kinase 1 Protein information
  • Uniprot ID CDK1_HUMAN
  • Uniprot AC P06493; A8K7C4; C9J497; O60764;
  • UniGene Hs.732435;
  • GeneID 983
  • KEGG hsa:983;
  • Human Cyclin-dependent kinase 1 Protein SEQUENCE
  • SEQUENCE 297 AA; 34095 MW; 942D79448EFE490A CRC64;

    MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR

    EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM

    DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA

    RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK

    KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA

    SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKM

  • UCSC uc001jld.3; human. [P06493-1];




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