Human PF4V1 ELISA Kit

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  • Alternative name

    Human C-X-C motif chemokine 4 variant ELISA Kit;Human CXCL4L1 ELISA Kit;Human PF4alt ELISA Kit;Human PF4var1 ELISA Kit;Human CXCL4V1 ELISA Kit;Human SCYB4V1 ELISA Kit;Human PF4-ALT ELISA Kit;Human PF4A ELISA Kit;Human platelet factor 4 variant 1 ELISA Kit;Human platelet factor 4 variant ELISA Kit;Human C-X-C motif chemokine 4 ELISA Kit;Human platelet factor 4, variant 1 (PF4-like) ELISA Kit;

  • Catalog
    E029710
  • species
    Human
  • GenePF4V1
  • Standard CurveHuman PF4V1 ELISA Kit
  • Other Species Mouse PF4V1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman PF4V1 ELISA Kit allows for the in vitro quantitative determination of PF4V1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Principle of the Assay: PF4V1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PF4V1. Standards or samples are then added to the microtiter plate wells and PF4V1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PF4V1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PF4V1 are added to each well to "sandwich" the PF4V1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PF4V1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF4V1 concentration in each sample is interpolated from this standard curve.
  • Human Platelet factor 4 variant(6-74) Protein information
  • Uniprot ID PF4V_HUMAN
  • Uniprot AC P10720; A1L4S0;
  • UniGene Hs.72933;
  • GeneID 5197
  • KEGG hsa:5197;
  • Human Platelet factor 4 variant(6-74) Protein SEQUENCE
  • SEQUENCE 104 AA; 11553 MW; 786FE2B288532E28 CRC64;

    MSSAARSRLT RATRQEMLFL ALLLLPVVVA FARAEAEEDG DLQCLCVKTT

    SQVRPRHITS LEVIKAGPHC PTAQLIATLK NGRKICLDLQ ALLYKKIIKE

    HLES

  • UCSC uc003hhg.2; human.;


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