Human TNFRSF8 ELISA Kit

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  • Alternative name

    Human CD30L receptor ELISA Kit;Human Ki-1 antigen ELISA Kit;Human lymphocyte activation antigen CD30 ELISA Kit;Human CD30 ELISA Kit;Human D1S166E ELISA Kit;Human Ki-1 ELISA Kit;Human TNF receptor superfamily member 8 ELISA Kit;Human tumor necrosis factor receptor superfamily member 8 ELISA Kit;Human cytokine receptor CD30 ELISA Kit;

  • Catalog
    E007417
  • species
    Human
  • GeneTNFRSF8
  • Standard CurveHuman TNFRSF8 ELISA Kit
  • Other Species Mouse Tnfrsf8 ELISA KitRat Tnfrsf8 ELISA Kit
  • SpecificityNatural and recombinant human CD30
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human CD30 in cell culture supernates, serum and plasm
  • Intended UseHuman TNFRSF8 ELISA Kit allows for the in vitro quantitative determination of TNFRSF8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human CD30 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD30 has been precoated onto 96-well plates. Standards(NSO, F19-K379) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD30 amount of sample captured in plate. Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
  • Human Tumor necrosis factor receptor superfamily member 8 Protein information
  • Uniprot ID TNR8_HUMAN
  • Uniprot AC P28908; B1AN79; B9EGD9; D3YTD8; Q6P4D9;
  • UniGene Hs.1314;
  • GeneID 943
  • KEGG hsa:943;
  • Human Tumor necrosis factor receptor superfamily member 8 Protein SEQUENCE
  • SEQUENCE 595 AA; 63747 MW; 7A407CC78A6E0BC8 CRC64;

    MRVLLAALGL LFLGALRAFP QDRPFEDTCH GNPSHYYDKA VRRCCYRCPM

    GLFPTQQCPQ RPTDCRKQCE PDYYLDEADR CTACVTCSRD DLVEKTPCAW

    NSSRVCECRP GMFCSTSAVN SCARCFFHSV CPAGMIVKFP GTAQKNTVCE

    PASPGVSPAC ASPENCKEPS SGTIPQAKPT PVSPATSSAS TMPVRGGTRL

    AQEAASKLTR APDSPSSVGR PSSDPGLSPT QPCPEGSGDC RKQCEPDYYL

    DEAGRCTACV SCSRDDLVEK TPCAWNSSRT CECRPGMICA TSATNSCARC

    VPYPICAAET VTKPQDMAEK DTTFEAPPLG TQPDCNPTPE NGEAPASTSP

    TQSLLVDSQA SKTLPIPTSA PVALSSTGKP VLDAGPVLFW VILVLVVVVG

    SSAFLLCHRR ACRKRIRQKL HLCYPVQTSQ PKLELVDSRP RRSSTQLRSG

    ASVTEPVAEE RGLMSQPLME TCHSVGAAYL ESLPLQDASP AGGPSSPRDL

    PEPRVSTEHT NNKIEKIYIM KADTVIVGTV KAELPEGRGL AGPAEPELEE

    ELEADHTPHY PEQETEPPLG SCSDVMLSVE EEGKEDPLPT AASGK

  • UCSC uc001atq.3; human. [P28908-1];




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