Human CCK 8 ELISA Kit

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  • Alternative name

    Cholecystokinin ELISA KIT; (1-49)-CCK58CCK ELISA KIT; CCK ELISA KIT; CCK58 ELISA KIT; CCK39 ELISA KIT; CCK33 ELISA KIT; CCK25 ELISA KIT; CCK18 ELISA KIT; CCK12 ELISA KIT; CCK8 ELISA KIT; CCK7 ELISA KIT; CCK5 ELISA KIT

  • Catalog
    E008211
  • species
    Human
  • GeneCCK 8
  • Standard CurveHuman CCK 8 ELISA Kit
  • Other Species Human CCK-8 ELISA KitMouse CCK-8 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman CCK 8 ELISA Kit allows for the in vitro quantitative determination of CCK 8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: CCK-8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCK-8 antibody and an CCK-8-HRP conjugate. The assay sample and buffer are incubated together with CCK-8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCK-8 concentration since CCK-8 from samples and CCK-8-HRP conjugate compete for the anti-CCK-8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCK-8 from the sample, fewer sites are left to bind CCK-8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCK-8 concentration in each sample is interpolated from this standard curve.




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