Mouse Wnt4 ELISA Kit

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  • Alternative name

    Mouse Wnt-4 ELISA Kit;Mouse wingless-type MMTV integration site family, member 4 ELISA Kit;Mouse protein Wnt-4 ELISA Kit;Mouse signal molecule ELISA Kit;Mouse wingless-related MMTV integration site 4 ELISA Kit;

  • Catalog
    E072778
  • species
    Mouse
  • GeneWnt4
  • Standard CurveMouse Wnt4 ELISA Kit
  • Other Species Human WNT4 ELISA KitChicken WNT4 ELISA KitTurkey WNT4 ELISA KitRat Wnt4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of WNT4. No significant cross-reactivity or interference between WNT4 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of WNT4 is typically less than 0.054ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
  • Intended UseMouse Wnt4 ELISA Kit allows for the in vitro quantitative determination of Wnt4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Protein Wnt-4 Protein information
  • Uniprot ID WNT4_MOUSE
  • Uniprot AC P22724;
  • UniGene Mm.20355;
  • GeneID 22417
  • KEGG mmu:22417;
  • Mouse Protein Wnt-4 Protein SEQUENCE
  • SEQUENCE 351 AA; 39050 MW; 7E1C5C739BE939D9 CRC64;

    MSPRSCLRSL RLLVFAVFSA AASNWLYLAK LSSVGSISEE ETCEKLKGLI

    QRQVQMCKRN LEVMDSVRRG AQLAIEECQY QFRNRRWNCS TLDSLPVFGK

    VVTQGTREAA FVYAISSAGV AFAVTRACSS GELEKCGCDR TVHGVSPQGF

    QWSGCSDNIA YGVAFSQSFV DVRERSKGAS SSRALMNLHN NEAGRKAILT

    HMRVECKCHG VSGSCEVKTC WRAVPPFRQV GHALKEKFDG ATEVEPRRVG

    SSRALVPRNA QFKPHTDEDL VYLEPSPDFC EQDIRSGVLG TRGRTCNKTS

    KAIDGCELLC CGRGFHTAQV ELAERCGCRF HWCCFVKCRQ CQRLVEMHTC

    R

  • UCSC uc008viv.2; mouse.;


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