Human PAPPA2 ELISA Kit

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  • Alternative name

    Human Pregnancy-associated plasma protein A2 ELISA Kit;Human PAPP-A2 ELISA Kit;Human pregnancy-associated plasma protein E1 ELISA Kit;Human PAPP-E ELISA Kit;Human PLAC3 ELISA Kit;Human PAPPE ELISA Kit;Human pappalysin 2 ELISA Kit;Human pappalysin-2 ELISA Kit;Human placenta-specific 3 ELISA Kit;Human pregnancy-associated plasma preproprotein-A2 ELISA Kit;

  • Catalog
    E028252
  • species
    Human
  • GenePAPPA2
  • Standard CurveHuman PAPPA2 ELISA Kit
  • Other Species Mouse PAPPA2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Pappalysin 2 (PAPPA2). No significant cross-reactivity or interference between Pappalysin 2 (PAPPA2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman PAPPA2 ELISA Kit allows for the in vitro quantitative determination of PAPPA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pappalysin 2 (PAPPA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pappalysin 2 (PAPPA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pappalysin 2 (PAPPA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pappalysin 2 (PAPPA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Pappalysin-2 Protein information
  • Uniprot ID PAPP2_HUMAN
  • Uniprot AC Q9BXP8; A9Z1Y8; Q96PH7; Q96PH8; Q9H4C9;
  • UniGene Hs.187284;
  • GeneID 60676
  • KEGG hsa:60676;
  • Human Pappalysin-2 Protein SEQUENCE
  • SEQUENCE 1791 AA; 198539 MW; F436030821EC6EDD CRC64;

    MMCLKILRIS LAILAGWALC SANSELGWTR KKSLVEREHL NQVLLEGERC

    WLGAKVRRPR ASPQHHLFGV YPSRAGNYLR PYPVGEQEIH HTGRSKPDTE

    GNAVSLVPPD LTENPAGLRG AVEEPAAPWV GDSPIGQSEL LGDDDAYLGN

    QRSKESLGEA GIQKGSAMAA TTTTAIFTTL NEPKPETQRR GWAKSRQRRQ

    VWKRRAEDGQ GDSGISSHFQ PWPKHSLKHR VKKSPPEESN QNGGEGSYRE

    AETFNSQVGL PILYFSGRRE RLLLRPEVLA EIPREAFTVE AWVKPEGGQN

    NPAIIAGVFD NCSHTVSDKG WALGIRSGKD KGKRDARFFF SLCTDRVKKA

    TILISHSRYQ PGTWTHVAAT YDGRHMALYV DGTQVASSLD QSGPLNSPFM

    ASCRSLLLGG DSSEDGHYFR GHLGTLVFWS TALPQSHFQH SSQHSSGEEE

    ATDLVLTASF EPVNTEWVPF RDEKYPRLEV LQGFEPEPEI LSPLQPPLCG

    QTVCDNVELI SQYNGYWPLR GEKVIRYQVV NICDDEGLNP IVSEEQIRLQ

    HEALNEAFSR YNISWQLSVH QVHNSTLRHR VVLVNCEPSK IGNDHCDPEC

    EHPLTGYDGG DCRLQGRCYS WNRRDGLCHV ECNNMLNDFD DGDCCDPQVA

    DVRKTCFDPD SPKRAYMSVK ELKEALQLNS THFLNIYFAS SVREDLAGAA

    TWPWDKDAVT HLGGIVLSPA YYGMPGHTDT MIHEVGHVLG LYHVFKGVSE

    RESCNDPCKE TVPSMETGDL CADTAPTPKS ELCREPEPTS DTCGFTRFPG

    APFTNYMSYT DDNCTDNFTP NQVARMHCYL DLVYQQWTES RKPTPIPIPP

    MVIGQTNKSL TIHWLPPISG VVYDRASGSL CGACTEDGTF RQYVHTASSR

    RVCDSSGYWT PEEAVGPPDV DQPCEPSLQA WSPEVHLYHM NMTVPCPTEG

    CSLELLFQHP VQADTLTLWV TSFFMESSQV LFDTEILLEN KESVHLGPLD

    TFCDIPLTIK LHVDGKVSGV KVYTFDERIE IDAALLTSQP HSPLCSGCRP

    VRYQVLRDPP FASGLPVVVT HSHRKFTDVE VTPGQMYQYQ VLAEAGGELG

    EASPPLNHIH GAPYCGDGKV SERLGEECDD GDLVSGDGCS KVCELEEGFN

    CVGEPSLCYM YEGDGICEPF ERKTSIVDCG IYTPKGYLDQ WATRAYSSHE

    DKKKCPVSLV TGEPHSLICT SYHPDLPNHR PLTGWFPCVA SENETQDDRS

    EQPEGSLKKE DEVWLKVCFN RPGEARAIFI FLTTDGLVPG EHQQPTVTLY

    LTDVRGSNHS LGTYGLSCQH NPLIINVTHH QNVLFHHTTS VLLNFSSPRV

    GISAVALRTS SRIGLSAPSN CISEDEGQNH QGQSCIHRPC GKQDSCPSLL

    LDHADVVNCT SIGPGLMKCA ITCQRGFALQ ASSGQYIRPM QKEILLTCSS

    GHWDQNVSCL PVDCGVPDPS LVNYANFSCS EGTKFLKRCS ISCVPPAKLQ

    GLSPWLTCLE DGLWSLPEVY CKLECDAPPI ILNANLLLPH CLQDNHDVGT

    ICKYECKPGY YVAESAEGKV RNKLLKIQCL EGGIWEQGSC IPVVCEPPPP

    VFEGMYECTN GFSLDSQCVL NCNQEREKLP ILCTKEGLWT QEFKLCENLQ

    GECPPPPSEL NSVEYKCEQG YGIGAVCSPL CVIPPSDPVM LPENITADTL

    EHWMEPVKVQ SIVCTGRRQW HPDPVLVHCI QSCEPFQADG WCDTINNRAY

    CHYDGGDCCS STLSSKKVIP FAADCDLDEC TCRDPKAEEN Q

  • UCSC uc001gky.2; human. [Q9BXP8-1];


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