Human CRK ELISA Kit

Alternative name
Human proto-oncogene c-Crk ELISA Kit;Human p38 ELISA Kit;Human CRKII ELISA Kit;Human CRK proto-oncogene, adaptor protein ELISA Kit;Human adapter molecule crk ELISA Kit;Human v-crk avian sarcoma virus CT10 oncogene homolog ELISA Kit;Human v-crk sarcoma virus CT10 oncogene-like protein ELISA Kit;
Catalog
E000719
species
Human
GeneCRK
Standard Curve
Other Species Mouse Crk ELISA Kit Chicken CRK ELISA Kit Rat Crk ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CRK. No significant cross-reactivity or interference between CRK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRK and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman CRK ELISA Kit allows for the in vitro quantitative determination of CRK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalPrinciple of the assay: CRK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRK antibody and an CRK-HRP conjugate. The assay sample and buffer are incubated together with CRK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRK concentration since CRK from samples and CRK-HRP conjugate compete for the anti-CRK antibody binding site. Since the number of sites is limited, as more sites are occupied by CRK from the sample, fewer sites are left to bind CRK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRK concentration in each sample is interpolated from this standard curve.
Human Adapter molecule crk Protein information
Uniprot ID CRK_HUMAN
Uniprot AC P46108; A8MWE8; B0LPE8; D3DTH6; Q96GA9; Q96HJ0;
UniGene Hs.461896;
GeneID 1398
KEGG hsa:1398;
Human Adapter molecule crk Protein SEQUENCE
SEQUENCE 304 AA; 33831 MW; 4CFBFB65BFC2E265 CRC64;
MAGNFDSEER SSWYWGRLSR QEAVALLQGQ RHGVFLVRDS STSPGDYVLS
VSENSRVSHY IINSSGPRPP VPPSPAQPPP GVSPSRLRIG DQEFDSLPAL
LEFYKIHYLD TTTLIEPVSR SRQGSGVILR QEEAEYVRAL FDFNGNDEED
LPFKKGDILR IRDKPEEQWW NAEDSEGKRG MIPVPYVEKY RPASASVSAL
IGGNQEGSHP QPLGGPEPGP YAQPSVNTPL PNLQNGPIYA RVIQKRVPNA
YDKTALALEV GELVKVTKIN VSGQWEGECN GKRGHFPFTH VRLLDQQNPD
EDFS
UCSC uc002fsl.4; human. [P46108-1];

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Human CRK ELISA Kit

Human proto-oncogene c-Crk ELISA Kit;Human p38 ELISA Kit;Human CRKII ELISA Kit;Human CRK proto-oncogene, adaptor protein ELISA Kit;Human adapter molecule crk ELISA Kit;Human v-crk avian sarcoma virus CT10 oncogene homolog ELISA Kit;Human v-crk sarcoma virus CT10 oncogene-like protein ELISA Kit;