Human CAV3 ELISA Kit

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  • Alternative name

    Human M-caveolin ELISA Kit;Human LGMD1C ELISA Kit;Human LQT9 ELISA Kit;Human VIP-21 ELISA Kit;Human VIP21 ELISA Kit;Human caveolin 3 ELISA Kit;Human caveolin-3 ELISA Kit;Human cavolin 3 ELISA Kit;

  • Catalog
    E007128
  • species
    Human
  • GeneCAV3
  • Standard CurveHuman CAV3 ELISA Kit
  • Other Species Mouse Cav3 ELISA KitBovine CAV3 ELISA KitPorcine CAV3 ELISA KitRat Cav3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CAV3. No significant cross-reactivity or interference between CAV3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAV3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CAV3 ELISA Kit allows for the in vitro quantitative determination of CAV3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: CAV3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAV3 antibody and an CAV3-HRP conjugate. The assay sample and buffer are incubated together with CAV3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAV3 concentration since CAV3 from samples and CAV3-HRP conjugate compete for the anti-CAV3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAV3 from the sample, fewer sites are left to bind CAV3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAV3 concentration in each sample is interpolated from this standard curve.
  • Human Caveolin-3 Protein information
  • Uniprot ID CAV3_HUMAN
  • Uniprot AC P56539; A8K777; Q3T1A4;
  • UniGene Hs.98303;
  • GeneID 859
  • KEGG hsa:859;
  • Human Caveolin-3 Protein SEQUENCE
  • SEQUENCE 151 AA; 17259 MW; C695E14F5B8F4753 CRC64;

    MMAEEHTDLE AQIVKDIHCK EIDLVNRDPK NINEDIVKVD FEDVIAEPVG

    TYSFDGVWKV SYTTFTVSKY WCYRLLSTLL GVPLALLWGF LFACISFCHI

    WAVVPCIKSY LIEIQCISHI YSLCIRTFCN PLFAALGQVC SSIKVVLRKE

    V

  • UCSC uc003bra.4; human.;


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