Human A1CF ELISA Kit

Alternative name
Human APOBEC1-stimulating protein ELISA Kit;Human ACF ELISA Kit;Human ASP ELISA Kit;Human ACF64 ELISA Kit;Human ACF65 ELISA Kit;Human APOBEC1CF ELISA Kit;Human APOBEC1 complementation factor ELISA Kit;Human APOBEC-1 stimulating protein ELISA Kit;Human apo-B RNA editing protein ELISA Kit;Human apobec-1 complementation factor (ACF) (ASP) ELISA Kit;
Catalog
E003248
species
Human
GeneA1CF
Other Species Mouse A1cf ELISA Kit Rat A1cf ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human A1CF. No significant cross-reactivity or interference between human A1CF and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human A1CF is typically less than 11.72 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman A1CF ELISA Kit allows for the in vitro quantitative determination of A1CF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for A1CF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any A1CF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A1CF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A1CF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human APOBEC1 complementation factor Protein information
Uniprot ID A1CF_HUMAN
Uniprot AC Q9NXC9; Q9NZD3;
UniGene Hs.282795;
GeneID 29974
KEGG hsa:29974;
Human APOBEC1 complementation factor Protein SEQUENCE
SEQUENCE 594 AA; 65202 MW; AA5EF76BD8815807 CRC64;
MESNHKSGDG LSGTQKEAAL RALVQRTGYS LVQENGQRKY GGPPPGWDAA
PPERGCEIFI GKLPRDLFED ELIPLCEKIG KIYEMRMMMD FNGNNRGYAF
VTFSNKVEAK NAIKQLNNYE IRNGRLLGVC ASVDNCRLFV GGIPKTKKRE
EILSEMKKVT EGVVDVIVYP SAADKTKNRG FAFVEYESHR AAAMARRKLL
PGRIQLWGHG IAVDWAEPEV EVDEDTMSSV KILYVRNLML STSEEMIEKE
FNNIKPGAVE RVKKIRDYAF VHFSNREDAV EAMKALNGKV LDGSPIEVTL
AKPVDKDSYV RYTRGTGGRG TMLQGEYTYS LGQVYDPTTT YLGAPVFYAP
QTYAAIPSLH FPATKGHLSN RAIIRAPSVR EIYMNVPVGA AGVRGLGGRG
YLAYTGLGRG YQVKGDKRED KLYDILPGME LTPMNPVTLK PQGIKLAPQI
LEEICQKNNW GQPVYQLHSA IGQDQRQLFL YKITIPALAS QNPAIHPFTP
PKLSAFVDEA KTYAAEYTLQ TLGIPTDGGD GTMATAAAAA TAFPGYAVPN
ATAPVSAAQL KQAVTLGQDL AAYTTYEVYP TFAVTARGDG YGTF
UCSC uc001jjh.4; human. [Q9NQ94-1];

Category

Human A1CF ELISA Kit