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Alternative name
Transferrin receptor protein 1 ELISA KIT; CD_antigen: CD71Tfrc ELISA KIT; Trfr ELISA KIT; TR ELISA KIT; TfR ELISA KIT; TfR1 ELISA KIT; Trfr ELISA KIT
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Catalog
E070668
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species
Mouse
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GeneTFRC
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse TFRC. No significant cross-reactivity or interference between Mouse TFRC and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.156 ng/ml
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Intended UseMouse TFRC ELISA Kit allows for the in vitro quantitative determination of TFRC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalFor Sample For the quantitative determination of mouse transferrin receptor (TFR) concentrations in serum, plasma, tissue homogenates.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
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