Human CSNK2A1 ELISA Kit

Alternative name
Human CK II alpha ELISA Kit;Human CK2A1 ELISA Kit;Human CKII ELISA Kit;Human CSNK2A3 ELISA Kit;Human OCNDS ELISA Kit;Human casein kinase 2 alpha 1 ELISA Kit;Human casein kinase II subunit alpha ELISA Kit;Human CK II alpha 3 ELISA Kit;Human CK2 catalytic subunit alpha ELISA Kit;Human casein kinase 2, alpha 1 polypeptide ELISA Kit;Human casein kinase II alpha 1 polypeptide pseudogene ELISA Kit;Human casein kinase II alpha 1 subunit ELISA Kit;Human protein kinase CK2 ELISA Kit;
Catalog
E006819
species
Human
GeneCSNK2A1
Standard Curve
Other Species Mouse Csnk2a1 ELISA Kit Bovine CSNK2A1 ELISA Kit Chicken CSNK2A1 ELISA Kit Rabbit CSNK2A1 ELISA Kit Rat Csnk2a1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CSNK2alpha1. No significant cross-reactivity or interference between CSNK2alpha1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CSNK2alpha1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman CSNK2A1 ELISA Kit allows for the in vitro quantitative determination of CSNK2A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This CSNK2alpha1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CSNK2alpha1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CSNK2alpha1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CSNK2alpha1 antibody and an CSNK2alpha1-HRP conjugate. The assay sample and buffer are incubated together with CSNK2alpha1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSNK2alpha1 concentration since CSNK2alpha1 from samples and CSNK2alpha1-HRP conjugate compete for the anti-CSNK2alpha1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CSNK2alpha1 from the sample, fewer sites are left to bind CSNK2alpha1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSNK2alpha1 concentration in each sample is interpolated from this standard curve.
Human Casein kinase II subunit alpha Protein information
Uniprot ID CSK21_HUMAN
Uniprot AC P68400; B4DYS6; D3DVV8; P19138; P20426; Q14013; Q5U065;
UniGene Hs.644056; Hs.654675; Hs.741126;
GeneID 1457
KEGG hsa:1457;
Human Casein kinase II subunit alpha Protein SEQUENCE
SEQUENCE 391 AA; 45144 MW; D3B6F5D13FF7422D CRC64;
MSGPVPSRAR VYTDVNTHRP REYWDYESHV VEWGNQDDYQ LVRKLGRGKY
SEVFEAINIT NNEKVVVKIL KPVKKKKIKR EIKILENLRG GPNIITLADI
VKDPVSRTPA LVFEHVNNTD FKQLYQTLTD YDIRFYMYEI LKALDYCHSM
GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE FYHPGQEYNV RVASRYFKGP
ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD QLVRIAKVLG
TEDLYDYIDK YNIELDPRFN DILGRHSRKR WERFVHSENQ HLVSPEALDF
LDKLLRYDHQ SRLTAREAME HPYFYTVVKD QARMGSSSMP GGSTPVSSAN
MMSGISSVPT PSPLGPLAGS PVIAAANPLG MPVPAAAGAQ Q
UCSC uc002wdw.2; human. [P68400-1];

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