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Catalog
E070466
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species
Mouse
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GeneT-IgG
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Other Species
Human T-IgG ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse T-IgG ELISA Kit allows for the in vitro quantitative determination of T-IgG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: For the qualitative determination of activated Mouse Toxoplasma Antibody IgG (TP-IgG) in serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.
Principle of the Assay||The kit uses sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Mouse TP-IgG in samples. Add sample to wells pre-coated with one Mouse Toxoplasma antigen, at same time add HRP-conjugated Mouse Toxoplasma IgG antibody to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Mouse TP-IgG in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.
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