SpecificityThe microplate is coated with highly purified ribosomal P protein (Rib-P) as antigen. The Anti- Rib-P test kits recognizes only autoantibodies specific to the phosphoproteins P0, P1 and P2 of the 60s subunit of ribosomal RNP complexes. No cross reactivities to other RNP epitopes and autoantigens have been observed.
SensitivityThe lower detection limit for Anti-Rib-P was determined at 1.0 U/ml.
Intended UseHuman Rib-P ELISA Kit allows for the in vitro quantitative determination of Rib-P , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyAutoimmune; Immunology; Research Assays; Assay Categories
Product Description specificalPrinciple of the assay: Highly purified ribosomal P protein (Rib-P) is bound to microwells. Antibodies against this antigen, if present in diluted serum or plasma, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow color is measured photometrically at 450 nm. The amount of color is directly proportional to the concentration of IgG antibodies present in the original sample.