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Alternative name
Tumor necrosis factor ELISA KIT; Cachectin ELISA KIT; TNF-alpha ELISA KIT; Tumor necrosis factor ligand superfamily member 2 ELISA KIT; TNF-aTNF ELISA KIT; TNFA ELISA KIT; TNFSF2 ELISA KIT; TNF-a ELISA KIT; NTF ELISA KIT; ICD1 ELISA KIT; ICD2 ELISA KIT
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Catalog
E070314
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species
Mouse
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GeneTNF- Alpha
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity3 pg/ml
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Intended UseMouse TNF- Alpha ELISA Kit allows for the in vitro quantitative determination of TNF- Alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology; Cytokines & Cell Signalling; Oncology; Research Assays; Assay Categories
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Product Description
specificalIntended Use The mouse TNF-? ELISA is an enzyme-linked immunosorbent assay for the detection of mouse TNF-?. The mouse TNF-? ELISA is for research use only. Not for use in diagnostic procedures..
Principle of the Test An anti-mouse TNF-? coating antibody is adsorbed onto microwells. Mouse TNF-? present in the sample orstandard binds to antibodies adsorbed to the microwells. Following incubation unbound biological components are removed during a wash step and a biotin-conjugated anti-mouse TNF-? antibody is added and binds to mouse TNF-? captured by the first antibody. Following incubation unbound biotinconjugated anti-mouse TNF-? antibody is removed during a wash step. Streptavidin- HRP is added and binds to the biotinconjugated anti-mouse TNF-? antibody. Following incubation unbound Streptavidin- HRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of mouse TNF-? present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 mouse TNF-? standard dilutions and mouse TNF-? sample concentration determined.
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