Human cAMP ELISA Kit

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  • Alternative name

    Cyclic adenosine monophosphate ELISA Kit

  • Catalog
    E006991
  • species
    Human
  • GenecAMP
  • Standard CurveHuman cAMP ELISA Kit
  • Other Species Human CAMP ELISA KitMouse cAMP ELISA KitMonkey cAMP ELISA KitBovine cAMP ELISA KitCanine cAMP ELISA KitChicken cAMP ELISA KitGeneral cAMP ELISA KitPorcine cAMP ELISA KitRabbit cAMP ELISA KitRat cAMP ELISA KitCamel cAMP ELISA KitGoat cAMP ELISA KitGuinea Pig cAMP ELISA KitHamster cAMP ELISA KitHorse cAMP ELISA KitSheep cAMP ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.938ng/ml
  • Intended UseHuman cAMP ELISA Kit allows for the in vitro quantitative determination of cAMP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    specifical
    Human cAMP ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to cAMP, During the reaction,cAMP in the sample or standard competes with a fixed amount of biotin-labeled cAMP for sites on a pre-coated Monoclonal antibody specific to cAMP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cAMP in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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