Intended UseMouse HBsAg ELISA Kit allows for the in vitro quantitative determination of HBsAg , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with HBsAb. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated HBsAb and incubated. Then substrate solutions are added to each well. Only those wells that contain HBsAg and enzyme-conjugated HBsAb will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.