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Catalog
E069902
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species
Mouse
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GeneTGFbeta1
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 2pg/ml
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Intended UseMouse TGFbeta1 ELISA Kit allows for the in vitro quantitative determination of TGFbeta1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TGFbeta1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TGFbeta1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGFbeta1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGFbeta1 can be calculated.
Background: Transforming growth factor beta 1 or TGFbeta1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. In humans, TGFbeta1 is encoded by the TGFB1 gene. This gene contains 7 exons and very large introns, maps to 19q13.1-q13.3. TGFbeta1 acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. The TGFB1 is directly involved in the pathogenesis of bone marrow reticulin fibrosis in hairy cell leukemia. The expression of TGFB1 in the early stages of DMD may be critical in initiating muscle fibrosis, and suggested that antifibrosis treatment might slow progression of the disease, increasing the utility of gene therapy.
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