Human ADCY1 ELISA Kit

Alternative name
Human ATP pyrophosphate-lyase 1 ELISA Kit;Human adenylate cyclase type I ELISA Kit;Human adenylyl cyclase 1 ELISA Kit;Human Ca(2+)/calmodulin-activated adenylyl cyclase ELISA Kit;Human AC1 ELISA Kit;Human DFNB44 ELISA Kit;Human adenylate cyclase 1 ELISA Kit;Human adenylate cyclase type 1 ELISA Kit;Human 3',5'-cyclic AMP synthetase ELISA Kit;Human adenyl cyclase ELISA Kit;Human adenylate cyclase 1 (brain) ELISA Kit;
Catalog
E000830
species
Human
GeneADCY1
Other Species Mouse Adcy1 ELISA Kit Bovine ADCY1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ADCY1 ELISA Kit allows for the in vitro quantitative determination of ADCY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: ADCY1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADCY1 antibody and an ADCY1-HRP conjugate. The assay sample and buffer are incubated together with ADCY1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADCY1 concentration since ADCY1 from samples and ADCY1-HRP conjugate compete for the anti-ADCY1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADCY1 from the sample, fewer sites are left to bind ADCY1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADCY1 concentration in each sample is interpolated from this standard curve.
Human Adenylate cyclase type 1 Protein information
Uniprot ID ADCY1_HUMAN
Uniprot AC Q08828; A4D2L8; Q75MI1;
UniGene Hs.192215;
GeneID 107
KEGG hsa:107;
Human Adenylate cyclase type 1 Protein SEQUENCE
SEQUENCE 1119 AA; 123440 MW; E8DDC8FCB58743D7 CRC64;
MAGAPRGGGG GGGGAGEPGG AERAAGTSRR RGLRACDEEF ACPELEALFR
GYTLRLEQAA TLKALAVLSL LAGALALAEL LGAPGPAPGL AKGSHPVHCV
LFLALLVVTN VRSLQVPQLQ QVGQLALLFS LTFALLCCPF ALGGPARGSA
GAAGGPATAE QGVWQLLLVT FVSYALLPVR SLLAIGFGLV VAASHLLVTA
TLVPAKRPRL WRTLGANALL FVGVNMYGVF VRILTERSQR KAFLQARSCI
EDRLRLEDEN EKQERLLMSL LPRNVAMEMK EDFLKPPERI FHKIYIQRHD
NVSILFADIV GFTGLASQCT AQELVKLLNE LFGKFDELAT ENHCRRIKIL
GDCYYCVSGL TQPKTDHAHC CVEMGLDMID TITSVAEATE VDLNMRVGLH
TGRVLCGVLG LRKWQYDVWS NDVTLANVME AAGLPGKVHI TKTTLACLNG
DYEVEPGYGH ERNSFLKTHN IETFFIVPSH RRKIFPGLIL SDIKPAKRMK
FKTVCYLLVQ LMHCRKMFKA EIPFSNVMTC EDDDKRRALR TASEKLRNRS
SFSTNVVYTT PGTRVNRYIS RLLEARQTEL EMADLNFFTL KYKHVEREQK
YHQLQDEYFT SAVVLTLILA ALFGLVYLLI FPQSVVVLLL LVFCICFLVA
CVLYLHITRV QCFPGCLTIQ IRTVLCIFIV VLIYSVAQGC VVGCLPWAWS
SKPNSSLVVL SSGGQRTALP TLPCESTHHA LLCCLVGTLP LAIFFRVSSL
PKMILLSGLT TSYILVLELS GYTRTGGGAV SGRSYEPIVA ILLFSCALAL
HARQVDIRLR LDYLWAAQAE EEREDMEKVK LDNRRILFNL LPAHVAQHFL
MSNPRNMDLY YQSYSQVGVM FASIPNFNDF YIELDGNNMG VECLRLLNEI
IADFDELMEK DFYKDIEKIK TIGSTYMAAV GLAPTSGTKA KKSISSHLST
LADFAIEMFD VLDEINYQSY NDFVLRVGIN VGPVVAGVIG ARRPQYDIWG
NTVNVASRMD STGVQGRIQV TEEVHRLLRR CPYHFVCRGK VSVKGKGEML
TYFLEGRTDG NGSQIRSLGL DRKMCPFGRA GLQGRRPPVC PMPGVSVRAG
LPPHSPGQYL PSAAAGKEA
UCSC uc003tne.5; human.;

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Human ADCY1 ELISA Kit