Human ADCY1 ELISA Kit

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  • Alternative name

    Human ATP pyrophosphate-lyase 1 ELISA Kit;Human adenylate cyclase type I ELISA Kit;Human adenylyl cyclase 1 ELISA Kit;Human Ca(2+)/calmodulin-activated adenylyl cyclase ELISA Kit;Human AC1 ELISA Kit;Human DFNB44 ELISA Kit;Human adenylate cyclase 1 ELISA Kit;Human adenylate cyclase type 1 ELISA Kit;Human 3',5'-cyclic AMP synthetase ELISA Kit;Human adenyl cyclase ELISA Kit;Human adenylate cyclase 1 (brain) ELISA Kit;

  • Catalog
    E000830
  • species
    Human
  • GeneADCY1
  • Other Species Mouse Adcy1 ELISA KitBovine ADCY1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ADCY1 ELISA Kit allows for the in vitro quantitative determination of ADCY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: ADCY1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADCY1 antibody and an ADCY1-HRP conjugate. The assay sample and buffer are incubated together with ADCY1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADCY1 concentration since ADCY1 from samples and ADCY1-HRP conjugate compete for the anti-ADCY1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADCY1 from the sample, fewer sites are left to bind ADCY1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADCY1 concentration in each sample is interpolated from this standard curve.
  • Human Adenylate cyclase type 1 Protein information
  • Uniprot ID ADCY1_HUMAN
  • Uniprot AC Q08828; A4D2L8; Q75MI1;
  • UniGene Hs.192215;
  • GeneID 107
  • KEGG hsa:107;
  • Human Adenylate cyclase type 1 Protein SEQUENCE
  • SEQUENCE 1119 AA; 123440 MW; E8DDC8FCB58743D7 CRC64;

    MAGAPRGGGG GGGGAGEPGG AERAAGTSRR RGLRACDEEF ACPELEALFR

    GYTLRLEQAA TLKALAVLSL LAGALALAEL LGAPGPAPGL AKGSHPVHCV

    LFLALLVVTN VRSLQVPQLQ QVGQLALLFS LTFALLCCPF ALGGPARGSA

    GAAGGPATAE QGVWQLLLVT FVSYALLPVR SLLAIGFGLV VAASHLLVTA

    TLVPAKRPRL WRTLGANALL FVGVNMYGVF VRILTERSQR KAFLQARSCI

    EDRLRLEDEN EKQERLLMSL LPRNVAMEMK EDFLKPPERI FHKIYIQRHD

    NVSILFADIV GFTGLASQCT AQELVKLLNE LFGKFDELAT ENHCRRIKIL

    GDCYYCVSGL TQPKTDHAHC CVEMGLDMID TITSVAEATE VDLNMRVGLH

    TGRVLCGVLG LRKWQYDVWS NDVTLANVME AAGLPGKVHI TKTTLACLNG

    DYEVEPGYGH ERNSFLKTHN IETFFIVPSH RRKIFPGLIL SDIKPAKRMK

    FKTVCYLLVQ LMHCRKMFKA EIPFSNVMTC EDDDKRRALR TASEKLRNRS

    SFSTNVVYTT PGTRVNRYIS RLLEARQTEL EMADLNFFTL KYKHVEREQK

    YHQLQDEYFT SAVVLTLILA ALFGLVYLLI FPQSVVVLLL LVFCICFLVA

    CVLYLHITRV QCFPGCLTIQ IRTVLCIFIV VLIYSVAQGC VVGCLPWAWS

    SKPNSSLVVL SSGGQRTALP TLPCESTHHA LLCCLVGTLP LAIFFRVSSL

    PKMILLSGLT TSYILVLELS GYTRTGGGAV SGRSYEPIVA ILLFSCALAL

    HARQVDIRLR LDYLWAAQAE EEREDMEKVK LDNRRILFNL LPAHVAQHFL

    MSNPRNMDLY YQSYSQVGVM FASIPNFNDF YIELDGNNMG VECLRLLNEI

    IADFDELMEK DFYKDIEKIK TIGSTYMAAV GLAPTSGTKA KKSISSHLST

    LADFAIEMFD VLDEINYQSY NDFVLRVGIN VGPVVAGVIG ARRPQYDIWG

    NTVNVASRMD STGVQGRIQV TEEVHRLLRR CPYHFVCRGK VSVKGKGEML

    TYFLEGRTDG NGSQIRSLGL DRKMCPFGRA GLQGRRPPVC PMPGVSVRAG

    LPPHSPGQYL PSAAAGKEA

  • UCSC uc003tne.5; human.;


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