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Mouse Tef ELISA Kit

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  • Alternative name

    Mouse 2310028D20Rik ELISA Kit;Mouse thyrotroph embryonic factor ELISA Kit;Mouse thyrotrophic embryonic factor ELISA Kit;
  • Catalog
    E069692
  • species
    Mouse
  • GeneTef
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse TEF. No significant cross-reactivity or interference between Mouse TEF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse TEF and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Tef ELISA Kit allows for the in vitro quantitative determination of Tef , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TEF in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TEF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse TEF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TEF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse TEF. You can calculate the concentration of Mouse TEF in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Thyrotroph embryonic factor Protein information
  • Uniprot ID TEF_MOUSE
  • Uniprot AC Q9JLC6; Q3U426; Q3UGF4; Q3UM49; Q3URC8; Q6QHT6; Q8C6I0; Q8VD02;
  • UniGene Mm.270278;
  • GeneID 21685
  • KEGG mmu:21685;
  • Mouse Thyrotroph embryonic factor Protein SEQUENCE

  • SEQUENCE 301 AA; 33145 MW; F83FFFC6D7E091A4 CRC64;

    MSDAGGGKKP PVEPQAGPGP GRAAGERGLS GSFPLVLKKL MENPPRETRL

    DKEKGKEKLE EDESAAASTM AVSASLMPPI WDKTIPYDGE SFHLEYMDLD

    EFLLENGIPA SPTHLAQNLL LPVAELEGKE SASSSTASPP SSSTAIFQPS

    ETVSSTESSL EKERETPSPI DPSCVEVDVN FNPDPADLVL SSVPGGELFN

    PRKHRFAEED LKPQPMIKKA KKVFVPDEQK DEKYWTRRKK NNVAAKRSRD

    ARRLKENQIT IRAAFLEKEN TALRTEVAEL RKEVGKCKTI VSKYETKYGP

    L

  • UCSC uc007wxk.2; mouse. [Q9JLC6-1];



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