Mouse Srpx2 ELISA Kit

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  • Alternative name

    Mouse 1110039C07Rik ELISA Kit;Mouse SRP ELISA Kit;Mouse SRPUL ELISA Kit;Mouse sushi-repeat-containing protein, X-linked 2 ELISA Kit;Mouse sushi repeat-containing protein SRPX2 ELISA Kit;Mouse sushi-repeat containing protein ELISA Kit;

  • Catalog
    E069254
  • species
    Mouse
  • GeneSrpx2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SRPX2. No significant cross-reactivity or interference between SRPX2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SRPX2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Srpx2 ELISA Kit allows for the in vitro quantitative determination of Srpx2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This SRPX2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SRPX2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||SRPX2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SRPX2 antibody and an SRPX2-HRP conjugate. The assay sample and buffer are incubated together with SRPX2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRPX2 concentration since SRPX2 from samples and SRPX2-HRP conjugate compete for the anti-SRPX2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SRPX2 from the sample, fewer sites are left to bind SRPX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRPX2 concentration in each sample is interpolated from this standard curve.
  • Mouse Sushi repeat-containing protein SRPX2 Protein information
  • Uniprot ID SRPX2_MOUSE
  • Uniprot AC Q8R054; B1AVI6; Q3UVU0; Q8K1F8; Q8K4W6;
  • UniGene Mm.263553;
  • GeneID 68792
  • KEGG mmu:68792;
  • Mouse Sushi repeat-containing protein SRPX2 Protein SEQUENCE
  • SEQUENCE 468 AA; 53140 MW; C48D645CC4B0DF3D CRC64;

    MMTSPLTQRG ALSLLLLLMP AVTPTWYAGS GYSPDESYNE VYAEEVPAAR

    ARALDYRVPR WCYTLNIQDG EATCYSPRGG NYHSSLGTRC ELSCDRGFRL

    IGRKSVQCLP SRRWSGTAYC RQIRCHTLPF ITSGTYTCTN GMLLDSRCDY

    SCSSGYHLEG DRSRICMEDG RWSGGEPVCV DIDPPKIRCP HSREKMAEPE

    KLTARVYWDP PLVKDSADGT ITRVTLRGPE PGSHFPEGEH VIRYTAYDRA

    YNRASCKFIV KVQVRRCPIL KPPQHGYLTC SSAGDNYGAI CEYHCDGGYE

    RQGTPSRVCQ SSRQWSGTPP VCTPMKINVN VNSAAGLLDQ FYEKQRLLIV

    SAPDPSNRYY KMQISMLQQS TCGLDLRHVT IIELVGQPPQ EVGRIREQQL

    SAGIIEELRQ FQRLTRSYFN MVLIDKQGID RERYMEPVTP EEIFTFIDDY

    LLSNEELARR VEQRDLCE

  • UCSC uc009ufd.3; mouse.;



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