Human CLOCK ELISA Kit

Alternative name
Human hCLOCK ELISA Kit;Human class E basic helix-loop-helix protein 8 ELISA Kit;Human bHLHe8 ELISA Kit;Human KIAA0334 ELISA Kit;Human KAT13D ELISA Kit;Human clock circadian regulator ELISA Kit;Human circadian locomoter output cycles protein kaput ELISA Kit;Human circadian locomoter output cycles kaput protein ELISA Kit;Human clock homolog ELISA Kit;
Catalog
E009657
species
Human
GeneCLOCK
SpecificityThis assay has high sensitivity and excellent specificity for detection of Clock Homolog (CLOCK). No significant cross-reactivity or interference between Clock Homolog (CLOCK) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.117ng/mL.
Intended UseHuman CLOCK ELISA Kit allows for the in vitro quantitative determination of CLOCK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Clock Homolog (CLOCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Clock Homolog (CLOCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Clock Homolog (CLOCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Clock Homolog (CLOCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Circadian locomoter output cycles protein kaput Protein information
Uniprot ID CLOCK_HUMAN
Uniprot AC O15516; A0AV01; A2I2N9; O14516; Q9UIT8;
UniGene Hs.436975; Hs.689578;
GeneID 9575
KEGG hsa:9575;
Human Circadian locomoter output cycles protein kaput Protein SEQUENCE
SEQUENCE 846 AA; 95304 MW; C292B451A33E4CBF CRC64;
MLFTVSCSKM SSIVDRDDSS IFDGLVEEDD KDKAKRVSRN KSEKKRRDQF
NVLIKELGSM LPGNARKMDK STVLQKSIDF LRKHKEITAQ SDASEIRQDW
KPTFLSNEEF TQLMLEALDG FFLAIMTDGS IIYVSESVTS LLEHLPSDLV
DQSIFNFIPE GEHSEVYKIL STHLLESDSL TPEYLKSKNQ LEFCCHMLRG
TIDPKEPSTY EYVKFIGNFK SLNSVSSSAH NGFEGTIQRT HRPSYEDRVC
FVATVRLATP QFIKEMCTVE EPNEEFTSRH SLEWKFLFLD HRAPPIIGYL
PFEVLGTSGY DYYHVDDLEN LAKCHEHLMQ YGKGKSCYYR FLTKGQQWIW
LQTHYYITYH QWNSRPEFIV CTHTVVSYAE VRAERRRELG IEESLPETAA
DKSQDSGSDN RINTVSLKEA LERFDHSPTP SASSRSSRKS SHTAVSDPSS
TPTKIPTDTS TPPRQHLPAH EKMVQRRSSF SSQSINSQSV GSSLTQPVMS
QATNLPIPQG MSQFQFSAQL GAMQHLKDQL EQRTRMIEAN IHRQQEELRK
IQEQLQMVHG QGLQMFLQQS NPGLNFGSVQ LSSGNSSNIQ QLAPINMQGQ
VVPTNQIQSG MNTGHIGTTQ HMIQQQTLQS TSTQSQQNVL SGHSQQTSLP
SQTQSTLTAP LYNTMVISQP AAGSMVQIPS SMPQNSTQSA AVTTFTQDRQ
IRFSQGQQLV TKLVTAPVAC GAVMVPSTML MGQVVTAYPT FATQQQQSQT
LSVTQQQQQQ SSQEQQLTSV QQPSQAQLTQ PPQQFLQTSR LLHGNPSTQL
ILSAAFPLQQ STFPQSHHQQ HQSQQQQQLS RHRTDSLPDP SKVQPQ
UCSC uc003haz.3; human.;

Category

Human CLOCK ELISA Kit