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Alternative name
Sterol regulatory element-binding protein 1 ELISA KIT; Sterol regulatory element-binding transcription factor 1Cleaved into the following chain:Processed sterol regulatory element-binding protein 1Srebf1 ELISA KIT; Srebp1 ELISA KIT; SREBP-1 ELISA KIT
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Catalog
E068997
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species
Mouse
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GeneSREBF1
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Other Species
Human SREBF1 ELISA KitPorcine SREBF1 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse SREBF1. No significant cross-reactivity or interference between Mouse SREBF1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity5.86 pg/ml
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Intended UseMouse SREBF1 ELISA Kit allows for the in vitro quantitative determination of SREBF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SREBF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SREBF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SREBF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SREBF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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