Human GPC2 ELISA Kit

Alternative name
Human glypican 2 ELISA Kit;Human glypican-2 ELISA Kit;Human cerebroglycan proteoglycan ELISA Kit;Human glypican proteoglycan 2, cerebroglycan proteoglycan ELISA Kit;
Catalog
E017217
species
Human
GeneGPC2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman GPC2 ELISA Kit allows for the in vitro quantitative determination of GPC2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: GPC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPC2 antibody and an GPC2-HRP conjugate. The assay sample and buffer are incubated together with GPC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPC2 concentration since GPC2 from samples and GPC2-HRP conjugate compete for the anti-GPC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPC2 from the sample, fewer sites are left to bind GPC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPC2 concentration in each sample is interpolated from this standard curve.
Human Secreted glypican-2 Protein information
Uniprot ID GPC2_HUMAN
Uniprot AC Q8N158; A4D2A7;
UniGene Hs.211701;
GeneID 221914
KEGG hsa:221914;
Human Secreted glypican-2 Protein SEQUENCE
SEQUENCE 579 AA; 62830 MW; 1630E3A22BB83DFA CRC64;
MSALRPLLLL LLPLCPGPGP GPGSEAKVTR SCAETRQVLG ARGYSLNLIP
PALISGEHLR VCPQEYTCCS SETEQRLIRE TEATFRGLVE DSGSFLVHTL
AARHRKFDEF FLEMLSVAQH SLTQLFSHSY GRLYAQHALI FNGLFSRLRD
FYGESGEGLD DTLADFWAQL LERVFPLLHP QYSFPPDYLL CLSRLASSTD
GSLQPFGDSP RRLRLQITRT LVAARAFVQG LETGRNVVSE ALKVPVSEGC
SQALMRLIGC PLCRGVPSLM PCQGFCLNVV RGCLSSRGLE PDWGNYLDGL
LILADKLQGP FSFELTAESI GVKISEGLMY LQENSAKVSA QVFQECGPPD
PVPARNRRAP PPREEAGRLW SMVTEEERPT TAAGTNLHRL VWELRERLAR
MRGFWARLSL TVCGDSRMAA DASLEAAPCW TGAGRGRYLP PVVGGSPAEQ
VNNPELKVDA SGPDVPTRRR RLQLRAATAR MKTAALGHDL DGQDADEDAS
GSGGGQQYAD DWMAGAVAPP ARPPRPPYPP RRDGSGGKGG GGSARYNQGR
SRSGGASIGF HTQTILILSL SALALLGPR
UCSC uc003utv.3; human.;

Category

Human GPC2 ELISA Kit

Human glypican 2 ELISA Kit;Human glypican-2 ELISA Kit;Human cerebroglycan proteoglycan ELISA Kit;Human glypican proteoglycan 2, cerebroglycan proteoglycan ELISA Kit;