Human GPC2 ELISA Kit

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  • Alternative name

    Human glypican 2 ELISA Kit;Human glypican-2 ELISA Kit;Human cerebroglycan proteoglycan ELISA Kit;Human glypican proteoglycan 2, cerebroglycan proteoglycan ELISA Kit;

  • Catalog
    E017217
  • species
    Human
  • GeneGPC2
  • Standard CurveHuman GPC2 ELISA Kit
  • Other Species Mouse Gpc2 ELISA KitRat Gpc2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GPC2 ELISA Kit allows for the in vitro quantitative determination of GPC2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: GPC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPC2 antibody and an GPC2-HRP conjugate. The assay sample and buffer are incubated together with GPC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPC2 concentration since GPC2 from samples and GPC2-HRP conjugate compete for the anti-GPC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPC2 from the sample, fewer sites are left to bind GPC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPC2 concentration in each sample is interpolated from this standard curve.
  • Human Secreted glypican-2 Protein information
  • Uniprot ID GPC2_HUMAN
  • Uniprot AC Q8N158; A4D2A7;
  • UniGene Hs.211701;
  • GeneID 221914
  • KEGG hsa:221914;
  • Human Secreted glypican-2 Protein SEQUENCE
  • SEQUENCE 579 AA; 62830 MW; 1630E3A22BB83DFA CRC64;

    MSALRPLLLL LLPLCPGPGP GPGSEAKVTR SCAETRQVLG ARGYSLNLIP

    PALISGEHLR VCPQEYTCCS SETEQRLIRE TEATFRGLVE DSGSFLVHTL

    AARHRKFDEF FLEMLSVAQH SLTQLFSHSY GRLYAQHALI FNGLFSRLRD

    FYGESGEGLD DTLADFWAQL LERVFPLLHP QYSFPPDYLL CLSRLASSTD

    GSLQPFGDSP RRLRLQITRT LVAARAFVQG LETGRNVVSE ALKVPVSEGC

    SQALMRLIGC PLCRGVPSLM PCQGFCLNVV RGCLSSRGLE PDWGNYLDGL

    LILADKLQGP FSFELTAESI GVKISEGLMY LQENSAKVSA QVFQECGPPD

    PVPARNRRAP PPREEAGRLW SMVTEEERPT TAAGTNLHRL VWELRERLAR

    MRGFWARLSL TVCGDSRMAA DASLEAAPCW TGAGRGRYLP PVVGGSPAEQ

    VNNPELKVDA SGPDVPTRRR RLQLRAATAR MKTAALGHDL DGQDADEDAS

    GSGGGQQYAD DWMAGAVAPP ARPPRPPYPP RRDGSGGKGG GGSARYNQGR

    SRSGGASIGF HTQTILILSL SALALLGPR

  • UCSC uc003utv.3; human.;


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