Human CASP5 ELISA Kit

Alternative name
Human CASP-5 ELISA Kit;Human ICE(rel)-III ELISA Kit;Human protease ICH-3 ELISA Kit;Human protease TY ELISA Kit;Human ICH3 ELISA Kit;Human ICE(rel)III ELISA Kit;Human ICEREL-III ELISA Kit;Human ICH-3 ELISA Kit;Human caspase 5 ELISA Kit;Human caspase-5 ELISA Kit;Human TY protease ELISA Kit;Human caspase 5, apoptosis-related cysteine peptidase ELISA Kit;Human caspase 5, apoptosis-related cysteine protease ELISA Kit;
Catalog
E006874
species
Human
GeneCASP5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Caspase 5 (CASP5). No significant cross-reactivity or interference between Caspase 5 (CASP5) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.063ng/mL.
Intended UseHuman CASP5 ELISA Kit allows for the in vitro quantitative determination of CASP5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 5 (CASP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 5 (CASP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 5 (CASP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 5 (CASP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Caspase-5 subunit p10 Protein information
Uniprot ID CASP5_HUMAN
Uniprot AC Q1HBJ3; Q6DJV7;
UniGene Hs.213327;
GeneID 838
KEGG hsa:838;
Human Caspase-5 subunit p10 Protein SEQUENCE
SEQUENCE 434 AA; 49736 MW; C5257C2BF15EB6D5 CRC64;
MAEDSGKKKR RKNFEAMFKG ILQSGLDNFV INHMLKNNVA GQTSIQTLVP
NTDQKSTSVK KDNHKKKTVK MLEYLGKDVL HGVFNYLAKH DVLTLKEEEK
KKYYDTKIED KALILVDSLR KNRVAHQMFT QTLLNMDQKI TSVKPLLQIE
AGPPESAEST NILKLCPREE FLRLCKKNHD EIYPIKKRED RRRLALIICN
TKFDHLPARN GAHYDIVGMK RLLQGLGYTV VDEKNLTARD MESVLRAFAA
RPEHKSSDST FLVLMSHGIL EGICGTAHKK KKPDVLLYDT IFQIFNNRNC
LSLKDKPKVI IVQACRGEKH GELWVRDSPA SLALISSQSS ENLEADSVCK
IHEEKDFIAF CSSTPHNVSW RDRTRGSIFI TELITCFQKY SCCCHLMEIF
RKVQKSFEVP QAKAQMPTIE RATLTRDFYL FPGN
UCSC uc010ruz.1; human. [P51878-1];

Category

Human CASP5 ELISA Kit