Human POMC ELISA Kit

Alternative name
Human POMC ELISA Kit;Human corticotropin-lipotropin ELISA Kit;Human gamma-MSH ELISA Kit;Human adrenocorticotropic hormone ELISA Kit;Human ACTH ELISA Kit;Human alpha-MSH ELISA Kit;Human CLIP ELISA Kit;Human beta-LPH ELISA Kit;Human gamma-LPH ELISA Kit;Human beta-MSH ELISA Kit;Human LPH ELISA Kit;Human MSH ELISA Kit;Human NPP ELISA Kit;Human POC ELISA Kit;Human proopiomelanocortin ELISA Kit;Human pro-opiomelanocortin ELISA Kit;Human adrenocorticotropin ELISA Kit;Human alpha-melanocyte-stimulating hormone ELISA Kit;Human beta-endorphin ELISA Kit;Human beta-melanocyte-stimulating hormone ELISA Kit;Human corticotropin-like intermediary peptide ELISA Kit;Human lipotropin beta ELISA Kit;Human lipotropin gamma ELISA Kit;Human melanotropin alpha ELISA Kit;Human melanotropin beta ELISA Kit;Human melanotropin gamma ELISA Kit;Human met-enkephalin ELISA Kit;Human opiomelanocortin prepropeptide ELISA Kit;Human pro-ACTH-endorphin ELISA Kit;Human proopiomelanocortin preproprotein ELISA Kit;
Catalog
E031187
species
Human
GenePOMC
SpecificityThis assay has high sensitivity and excellent specificity for detection of POMC. No significant cross-reactivity or interference between POMC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between POMC and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman POMC ELISA Kit allows for the in vitro quantitative determination of POMC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: POMC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-POMC antibody and an POMC-HRP conjugate. The assay sample and buffer are incubated together with POMC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the POMC concentration since POMC from samples and POMC-HRP conjugate compete for the anti-POMC antibody binding site. Since the number of sites is limited, as more sites are occupied by POMC from the sample, fewer sites are left to bind POMC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The POMC concentration in each sample is interpolated from this standard curve.
Human Met-enkephalin Protein information
Uniprot ID COLI_HUMAN
Uniprot AC P01189; P78442; Q53T23; Q9UD39; Q9UD40;
UniGene Hs.1897;
GeneID 5443
KEGG hsa:5443;
Human Met-enkephalin Protein SEQUENCE
SEQUENCE 267 AA; 29424 MW; B927323474A67536 CRC64;
MPRSCCSRSG ALLLALLLQA SMEVRGWCLE SSQCQDLTTE SNLLECIRAC
KPDLSAETPM FPGNGDEQPL TENPRKYVMG HFRWDRFGRR NSSSSGSSGA
GQKREDVSAG EDCGPLPEGG PEPRSDGAKP GPREGKRSYS MEHFRWGKPV
GKKRRPVKVY PNGAEDESAE AFPLEFKREL TGQRLREGDG PDGPADDGAG
AQADLEHSLL VAAEKKDEGP YRMEHFRWGS PPKDKRYGGF MTSEKSQTPL
VTLFKNAIIK NAYKKGE
UCSC uc002rfy.1; human.;

Category

Human POMC ELISA Kit