Mouse Sesn1 ELISA Kit

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  • Alternative name

    Mouse 1110002G11Rik ELISA Kit;Mouse AU044290 ELISA Kit;Mouse Pa26 ELISA Kit;Mouse Sest1 ELISA Kit;Mouse sestrin 1 ELISA Kit;Mouse sestrin-1 ELISA Kit;Mouse p53 regulated Pa26 nuclear protein ELISA Kit;Mouse p53-regulated protein PA26 ELISA Kit;

  • Catalog
    E067555
  • species
    Mouse
  • GeneSesn1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse SESN1. No significant cross-reactivity or interference between Mouse SESN1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse SESN1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse Sesn1 ELISA Kit allows for the in vitro quantitative determination of Sesn1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SESN1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SESN1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse SESN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SESN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse SESN1. You can calculate the concentration of Mouse SESN1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Sestrin-1 Protein information
  • Uniprot ID SESN1_MOUSE
  • Uniprot AC P58006; E9QPK0; Q7TNF3;
  • UniGene Mm.139418;
  • GeneID 140742
  • KEGG mmu:140742;
  • Mouse Sestrin-1 Protein SEQUENCE
  • SEQUENCE 492 AA; 56637 MW; 1DF5CF63A45F52F2 CRC64;

    MRLAAASNEA YAASLAVSEL LSCHQCGGDR GQDEELGIRI PRPLGHGPSR

    FIPEKEMLQV GSEDAQMHAL FADSFAALGR LDNITLVMVF HPQYLESFLK

    TQHYLLQMDG PLPLHYRHYI GIMAAARHQC SYLVNLHVSD FLHVGGDPKW

    LNGLENAPQK LQNLGELNKV LAHRPWLITK EHIEGLLKAE EHSWSLAELV

    HAVVLLTHYH SLASFTFGCG ISPEIHCDGG HTFRPPSVSN YCICDITNGN

    HSVDEMQVNS AGNASVSDSF FEVEALMEKM RQLQECREEE EASQEEMASR

    FEMEKRESMF VFSSDDDEVT PARDVSRHFE DTSYGYKDFS RHGMHVPTFR

    VQDYCWEDHG YSLVNRLYPD VGQLIDEKFH IAYNLTYNTM AMHKDVDTSM

    LRRAIWNYIH CMFGIRYDDY DYGEINQLLD RSFKVYIKTV VCTPEKVTKR

    MYDSFWRQFK HSEKVHVNLL LIEARMQAEL LYALRAITRY MT

  • UCSC uc007eyf.2; mouse.;



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