Human EML2 ELISA Kit

Alternative name
Human EMAP-2 ELISA Kit;Human HuEMAP-2 ELISA Kit;Human EMAP2 ELISA Kit;Human EMAPL2 ELISA Kit;Human ELP70 ELISA Kit;Human echinoderm microtubule associated protein like 2 ELISA Kit;Human echinoderm microtubule-associated protein-like 2 ELISA Kit;Human echinoderm MT-associated protein (EMAP)-like protein 70 ELISA Kit;Human microtubule-associated protein like echinoderm EMAP ELISA Kit;
Catalog
E012718
species
Human
GeneEML2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). No significant cross-reactivity or interference between Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.113ng/mL.
Intended UseHuman EML2 ELISA Kit allows for the in vitro quantitative determination of EML2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Echinoderm Microtubule Associated Protein Like Protein 2 (EML2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Echinoderm microtubule-associated protein-like 2 Protein information
Uniprot ID EMAL2_HUMAN
Uniprot AC O95834; B7Z3I2; B7Z3Q9; K7ERL7; Q59EN8; Q8N5A2; Q9UG50;
UniGene Hs.24178;
GeneID 24139
KEGG hsa:24139;
Human Echinoderm microtubule-associated protein-like 2 Protein SEQUENCE
SEQUENCE 649 AA; 70679 MW; F356D3D974D208C9 CRC64;
MSSFGAGKTK EVIFSVEDGS VKMFLRGRPV PMMIPDELAP TYSLDTRSEL
PSCRLKLEWV YGYRGRDCRA NLYLLPTGEI VYFVASVAVL YSVEEQRQRH
YLGHNDDIKC LAIHPDMVTI ATGQVAGTTK EGKPLPPHVR IWDSVSLSTL
HVLGLGVFDR AVCCVGFSKS NGGNLLCAVD ESNDHMLSVW DWAKETKVVD
VKCSNEAVLV ATFHPTDPTV LITCGKSHIY FWTLEGGSLS KRQGLFEKHE
KPKYVLCVTF LEGGDVVTGD SGGNLYVWGK GGNRITQAVL GAHDGGVFGL
CALRDGTLVS GGGRDRRVVL WGSDYSKLQE VEVPEDFGPV RTVAEGHGDT
LYVGTTRNSI LQGSVHTGFS LLVQGHVEEL WGLATHPSRA QFVTCGQDKL
VHLWSSDSHQ PLWSRIIEDP ARSAGFHPSG SVLAVGTVTG RWLLLDTETH
DLVAIHTDGN EQISVVSFSP DGAYLAVGSH DNLVYVYTVD QGGRKVSRLG
KCSGHSSFIT HLDWAQDSSC FVTNSGDYEI LYWDPATCKQ ITSADAVRNM
EWATATCVLG FGVFGIWSEG ADGTDINAVA RSHDGKLLAS ADDFGKVHLF
SYPCCQPRAL SHKYGGHSSH VTNVAFLWDD SMALTTGGKD TSVLQWRVV
UCSC uc002pcn.4; human. [O95834-1];

Category

Human EML2 ELISA Kit