Mouse Sema3f ELISA Kit

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  • Alternative name

    Mouse semaphorin IV ELISA Kit;Mouse Sema IV ELISA Kit;Mouse Sema4 ELISA Kit;Mouse Semak ELISA Kit;Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F ELISA Kit;Mouse semaphorin-3F ELISA Kit;

  • Catalog
    E067244
  • species
    Mouse
  • GeneSema3f
  • Standard CurveMouse Sema3f ELISA Kit
  • Other Species Human SEMA3F ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse SEMA3F. No significant cross-reactivity or interference between mouse SEMA3F and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse SEMA3F is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
  • Intended UseMouse Sema3f ELISA Kit allows for the in vitro quantitative determination of Sema3f , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3F is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3F bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse Semaphorin-3F Protein information
  • Uniprot ID SEM3F_MOUSE
  • Uniprot AC O88632; E9QNC5; O88633;
  • UniGene Mm.12903;
  • GeneID 20350
  • KEGG mmu:20350;
  • Mouse Semaphorin-3F Protein SEQUENCE
  • SEQUENCE 785 AA; 88549 MW; 8F3F0935096C24A1 CRC64;

    MLVTAFILWA SLLTGAWPAT PIQDQLPATP RVRLSFKELK ATGTAHFFNF

    LLNTTDYRIL LKDEDHDRMY VGSKDYVLSL DLHDINREPL IIHWAASPQR

    IEECILSGKD GNGECGNFVR LIQPWNRTHL YVCGTGAYNP MCTYVNRGRR

    AQALPWTQMQ VVRGRGSRAT DGADRPTPTA PRQDYIFYLE PEKLESGKGK

    CPYDPKLDTA SALINEELYA GVYIDFMGTD AAIFRTLGKQ TAMRTDQYNS

    RWLNDPSFIH AELIPDSAER NDDKLYFFFR ERSAEAPQNP AVYARIGRIC

    LNDDGGHCCL VNKWSTFLKA RLVCSVPGED GIETHFDELQ DVFVQQTQDV

    RNPVIYAVFT SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF

    SGKMPYPRPG TCPGGTFTPS MKSTKDYPDE VINFMRTHPL MYQAVYPLQR

    RPLVVRTGAP YRLTTVAVDQ VDAADGRYEV LFLGTDRGTV QKVIVLPKDD

    QEVEELMLEE VEVFKEPAPV KTMTISSKRQ QLYVASAVGV THLSLHRCQA

    YGAACADCCL ARDPYCAWDG QACSRYTASS KRRSRRQDVR HGNPIRQCRG

    FNSNANKNAV ESVQYGVAGS AAFLECQPRS PQATVKWLFQ RDPSDRRREI

    RAEDRFLRTE QGLLLRALQL GDRGLYSCTA TENNFKHIVT RVQLHVLGRD

    AVHAALFPPL AVSVPPPPGT GPPTPPYQEL AQLLAQPEVG LIHQYCQGYW

    RHVPPRPREA PGALRPPELQ DQKKPRNRRH HPPDT

  • UCSC uc009rmt.1; mouse. [O88632-2];



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