Human ERCC1 ELISA Kit

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  • Alternative name

    Human COFS4 ELISA Kit;Human RAD10 ELISA Kit;Human UV20 ELISA Kit;Human ERCC excision repair 1, endonuclease non-catalytic subunit ELISA Kit;Human DNA excision repair protein ERCC-1 ELISA Kit;Human excision repair cross-complementation group 1 ELISA Kit;Human excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) ELISA Kit;

  • Catalog
    E013795
  • species
    Human
  • GeneERCC1
  • Standard CurveHuman ERCC1 ELISA Kit
  • Other Species Mouse Ercc1 ELISA KitBovine ERCC1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1). No significant cross-reactivity or interference between Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.056ng/mL.
  • Intended UseHuman ERCC1 ELISA Kit allows for the in vitro quantitative determination of ERCC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human DNA excision repair protein ERCC-1 Protein information
  • Uniprot ID ERCC1_HUMAN
  • Uniprot AC P07992; B2RC01; B3KRR0; Q7Z7F5; Q96S40;
  • UniGene Hs.435981;
  • GeneID 2067
  • KEGG hsa:2067;
  • Human DNA excision repair protein ERCC-1 Protein SEQUENCE
  • SEQUENCE 297 AA; 32562 MW; 6FCE3615732349E5 CRC64;

    MDPGKDKEGV PQPSGPPARK KFVIPLDEDE VPPGVAKPLF RSTQSLPTVD

    TSAQAAPQTY AEYAISQPLE GAGATCPTGS EPLAGETPNQ ALKPGAKSNS

    IIVSPRQRGN PVLKFVRNVP WEFGDVIPDY VLGQSTCALF LSLRYHNLHP

    DYIHGRLQSL GKNFALRVLL VQVDVKDPQQ ALKELAKMCI LADCTLILAW

    SPEEAGRYLE TYKAYEQKPA DLLMEKLEQD FVSRVTECLT TVKSVNKTDS

    QTLLTTFGSL EQLIAASRED LALCPGLGPQ KARRLFDVLH EPFLKVP

  • UCSC uc002pbs.3; human. [P07992-1];


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