Mouse Pdhx ELISA Kit

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  • Alternative name

    Mouse Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex ELISA Kit;Mouse lipoyl-containing pyruvate dehydrogenase complex component X ELISA Kit;Mouse AI481367 ELISA Kit;Mouse E3bp ELISA Kit;Mouse Pdx1 ELISA Kit;Mouse pyruvate dehydrogenase complex, component X ELISA Kit;Mouse pyruvate dehydrogenase protein X component, mitochondrial ELISA Kit;Mouse dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex (E3-binding protein) (proX) ELISA Kit;

  • Catalog
    E065667
  • species
    Mouse
  • GenePdhx
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse PDHX. No significant cross-reactivity or interference between mouse PDHX and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse PDHX is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseMouse Pdhx ELISA Kit allows for the in vitro quantitative determination of Pdhx , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDHX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDHX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHX is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHX bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse Pyruvate dehydrogenase protein X component, mitochondrial Protein information
  • Uniprot ID ODPX_MOUSE
  • Uniprot AC Q8BKZ9;
  • UniGene Mm.315011;
  • GeneID 27402
  • KEGG mmu:27402;
  • Mouse Pyruvate dehydrogenase protein X component, mitochondrial Protein SEQUENCE
  • SEQUENCE 501 AA; 53999 MW; 3FD1FA752EAC5092 CRC64;

    MAASWRLHCN QPLLRYLLGF SSRRSLGLAQ GAAAWPVDRG ASWRWFHSTQ

    LLQADPIKVL MPSLSPTMEQ GNIVKWLRKE GEAVSAGDSL CEIETDKAVV

    TLDANDDGIL AKIVVEEGAK NIQLGSLIAL MVEEGEDWKQ VEIPKDVSAP

    PPVSKPPAPT QPSPQPQIPC PARKEHKGTA RFRLSPAARN ILEKHSLDAS

    QGTATGPRGI FTKEDALKLV ELKQMGKITE SRPASAPPPS LSASVPPQAT

    AGPSYPRPMT PPVSIPGQPN AAGTFTEIPA SNIRRVIAKR LTESKSTVPH

    AYATADCDLG AVLKVRRDLV KDDIKVSVND FIIRAAAVTL KQMPGVNVTW

    DGEGPKQLPS VDISVAVATD KGLITPIIKD AAAKGIQEIA DSVKVLSKKA

    RDGKLMPEEY QGGSFSISNL GMFGIDEFAA VINPPQACIL AVGRFRPVLK

    LTEDEEGNPQ LQQHQLITVT MSSDSRVVDD ELATRFLETF KANLENPMRL

    G

  • UCSC uc008lil.3; mouse.;


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