Human DDAH2 ELISA Kit

Alternative name
Human DDAH-2 ELISA Kit;Human dimethylarginine dimethylaminohydrolase 2 ELISA Kit;Human DDAHII ELISA Kit;Human dimethylargininase-2 ELISA Kit;Human Protein G6a ELISA Kit;Human S-phase protein ELISA Kit;Human DDAH ELISA Kit;Human G6a ELISA Kit;Human NG30 ELISA Kit;Human HEL-S-277 ELISA Kit;Human N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 ELISA Kit;Human dimethylarginine dimethylaminohydrolase II ELISA Kit;Human epididymis secretory protein Li 277 ELISA Kit;Human testis tissue sperm-binding protein Li 54e ELISA Kit;
Catalog
E011976
species
Human
GeneDDAH2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dimethylarginine Dimethylaminohydrolase 2 (DDAH2). No significant cross-reactivity or interference between Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.116ng/mL.
Intended UseHuman DDAH2 ELISA Kit allows for the in vitro quantitative determination of DDAH2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dimethylarginine Dimethylaminohydrolase 2 (DDAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 Protein information
Uniprot ID DDAH2_HUMAN
Uniprot AC O95865; A2BEZ7;
UniGene Hs.247362;
GeneID 23564
KEGG hsa:23564;
Human N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 Protein SEQUENCE
SEQUENCE 285 AA; 29644 MW; B3D9B00F29492548 CRC64;
MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG
KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV
DGVRKALQDL GLRIVEIGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE
IVADTFRDFA VSTVPVSGPS HLRGLCGMGG PRTVVAGSSD AAQKAVRAMA
VLTDHPYASL TLPDDAAADC LFLRPGLPGV PPFLLHRGGG DLPNSQEALQ
KLSDVTLVPV SCSELEKAGA GLSSLCLVLS TRPHS

Human DDAH2 ELISA Kit

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