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Alternative name
Pulmonary surfactant-associated protein D ELISA KIT; Collectin-7 ELISA KIT; Lung surfactant protein DSFTPD ELISA KIT; COLEC7 ELISA KIT; PSPD ELISA KIT; SFTP4 ELISA KIT; PSP-D ELISA KIT; SP-D ELISA KIT
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Catalog
E065557
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species
Mouse
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GeneSP-D
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse SP-D. No significant cross-reactivity or interference between mouse SP-D and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse SP-D is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
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Intended UseMouse SP-D ELISA Kit allows for the in vitro quantitative determination of SP-D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-D bound in the initial step. The color development is stopped and the intensity of the color is measured.
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